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Blood Cultures

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Megan Brashear, CVT, VTS (ECC), demonstrates the proper preparation and procedure for collecting blood cultures performed in a dog hospitalized for a fever of unknown origin.

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Megan Brashear's picture
Megan Brashear

CVT VTS(ECC)

Enrolled: 07/2011

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Laura Ryan's picture

Our clinic always pulls enough blood for both vials at the same time. What is the advantage of waiting an hour to pull for the second vial?

Thanks,
Ryan

Megan Brashear's picture

Hi Ryan,
By both vials I am assuming you're talking about aerobic and anaerobic which is a slightly different issue. Because blood cultures can be tricky to determine if the organism that is grown is from the blood and not from the skin or other contaminant, and because bacteria can be shed into the blood at different time intervals, we draw two separate cultures from two separate vessels an hour apart. If the lab grows the same organism from both separate cultures, we can be more confident that it is indeed from the blood. This depends on your hospital protocol, if you are having success then keep it up!

Kristen Black's picture

What was the medium used in the blood culture? Also, did the cultures come back with anything interesting on this patient?

Megan Brashear's picture

Kristen, here we use Oxoid Signal Blood Culture bottles, ordered through our outside lab. In this particular patient they did not grow anything exciting or interesting and he did well!