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Fecal Float and Fecal Direct Smear Setup

Views: 25099 - Comments: 11

Megan Brashear, CVT, VTS(ECC), demonstrates how to obtain a fecal sample, set up a fecal float and direct fecal examination.

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Megan Brashear's picture
Megan Brashear


Enrolled: 07/2011

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Thomas Taormina's picture

Nice demonstration, but I do believe the fecal float directions suggest 15 to 20 minutes bedsore examining your slide. This allows the eggs (especially smaller ones i.e coccidiosis) ample time to surface. 10 minute wait time could lead to unnecessary finding no parasites and/or ova.

Megan Brashear's picture

Thank yout for your comment Thomas, if you are using a sugar solution as your float medium, yes, the float time should be 20min. A 10min wait time should be the MINIMUM amount of time you leave it to give any ova a chance to float. Longer wait times are fine, as long as you don't wait too long so that the float medium starts to crystallize on the cover slip.

Victor Cantergiani's picture

Hi Megan. If a fecal float is forgotten and left sitting for a long time, how long would be too long? Would you just go by a visual inspection of the crystallization?

Ana Jordache's picture

One also Trick to view "bad bacteria" i.e clostridium/rods, is to do a wright stain smear. You can also identify giardia cyst that way.

Megan Brashear's picture

Thanks Ana, so true! Everything looks more pretty after a wright's stain! Those of you staining fecal slides are probably already doing this, but please separate your fecal stain jars from your CBC and other cytology stain jars so there's no cross contamination. Because poop is gross...

Andrea Dobson's picture

I'd love to see you do a demo on a sugar solution fecal centrifugation procedure-that is what we teach our VT students as the gold standard, and I am finding more and more practices are gravitating (no pun intended) to that method vs. the passive float.

Donald Howell's picture

Would like to see zinc sulfate solution used and swing arm centriguation .