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Major Crossmatch

Views: 14744 - Comments: 11

Megan Brashear, CVT, VTS (ECC), demonstrates how to perform a major crossmatch prior to administering a blood transfusion. By matching the recipient to a specific donor, you can significantly decrease the chances of a transfusion reaction.

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Megan Brashear's picture
Megan Brashear


Enrolled: 07/2011

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Zachary Mitchell's picture

Very informative video! I will use the procedure checklist for our in house training manual! Thank You.

Randi Metcalf's picture

What amount do you recommend to start your donor sample with? Also do you add your first saline wash directly to unseperated donor whole blood sample?

Jill Greene's picture

Hi Randi,
You should use at least 2mls of donor blood cells to start the crossmatch. You want to crossmatch to what your recipient is going to receive - either packed red blood cells, whole blood, or plasma. You run a major crossmatch for packed red blood cells. A minor crossmatch for plasma. And ideally both major and minor for whole blood.

Randi Metcalf's picture

After rewatching this video I noticed that at about 1:15 when talking about the major crossmatch she mentions using serum and then at 2:06 it states that with a minor crossmatch you use plasma. Is it true that you need different sample depending on the crossmatch you are performing?

Jill Greene's picture

Kind of?
A major crossmatch is for donor red cells into your patient. You can use either serum or plasma from your patient to mix with the donor's blood. Megan used serum for this video.
A minor crossmatch is for donor plasma into your patient. You would use a lavender top tube from your patient mixed with plasma from your donor. You will always be transfusing plasma, never serum.

Randi Metcalf's picture

Also at the end of the washed RBC cycle, when she adds the 4mls of saline she states that she now has a plasma mix?

Brandy Olaguez's picture

So if I crossmatch a cat to two units and they are both matches, is it recommended that we crossmatch again prior to the second unit just because of the introduction of new antibodies?

Kelsey Reinauer's picture

Hi Brandy,
Great question! If you are going to be using the second unit within 4 days of the first transfusion then you do not need to crossmatch again. However, after that 4 day mark you would want to, just in case the cat has developed new antibodies after that first transfusion. Hope this helps!