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Saline Agglutination Test

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Megan Brashear, CVT, VTS (ECC), explains the reasoning and demonstrates the process of performing and interpreting a saline agglutination test.

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Megan Brashear's picture
Megan Brashear


Enrolled: 07/2011

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Liz Hughston's picture

In my research, I've found different protocols for this test and some say to use fresh whole blood and some say to only use anticoagulated blood. Do you have a reference for your protocol?

Megan Brashear's picture

Sure Liz, here are three texts stating to use anticoagulated blood instead of fresh whole blood. While not stated anywhere, I make it a priority to use blood within 2-4 hours from the draw so it hasn't been sitting in the refrigerator or the counter for hours and the cells begin to do weird things. I try to use fresh anticoagulated blood.

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Ford, Richard B., DVM, MS, and Elisa M. Mazzaferro, MS, DVM, PhD. Kirk and Bistner's Handbook of Veterinary Procedures and Emergency Treatment. 8th ed. Philadelphia: W.B. Saunders, 2006. 609. Print.