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Staining a Blood Smear

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Megan Brashear, CVT, VTS (ECC), demonstrates technique in staining a blood smear for microscopic evaluation.

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Megan Brashear's picture
Megan Brashear

CVT VTS(ECC)

Enrolled: 07/2011

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Lindsey Kovarick's picture

About how long do you recommend leaving the slide in the purple stain when looking for platelets?

Megan Brashear's picture

Hi Lindsay, if I know I am only looking for platelets (and not doing a complete differential) I will leave the smear in the purple stain for at least 30 seconds. It will help those platelets to really show up well, but also may distort your other cells. If you're having trouble locating platelets you can make two smears and leave one in the stain longer for your platelets.